Not known Facts About principle of hplc and gc
The stationary phase is hydrophobic, While the functional stage is often a polar liquid, including h2o-methanol or acetonitrile mixes. It relies on the hydrophobic collaboration rule, which states that the far more nonpolar the substance, the more it will be retained.HPLC can only Assess substances which have been dissolved in solvents. HPLC separa